sequences for pyf515 Search Results


sequences for pyf515  (Plasmidsaurus)
86
Plasmidsaurus sequences for pyf515
Gel electrophoresis of products from a PCR performed on DNA from P. ramorum NA2_17 wildtype (PrWT; wells 3-4), <t>pYF515</t> transformant (pYF515 PrT; wells 5-7), and pYF2-PsCG transformant (pYF2-PsCG PrT; wells 10-14) cultures using the pYF_Cas9_Diag_F3/R3 primer pair (expected product size is 824 bp). The positive PCR controls are pYF515 plasmid DNA (well 2) and pYF2-PsCG plasmid DNA (well 9). The gel was run at 100 volts for 60 minutes on 0.8% agarose in 0.5X TBE buffer with a 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, MA, USA). All samples were loaded with Safe-Green™ stain (Applied Biological Materials Inc., Richmond, BC, Canada) at a ratio of 1 μL Safe-Green: 5 μL PCR product.
Sequences For Pyf515, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequences+for+pyf515/bio_rxiv__2024__06__13__598956-99-8-4?v=Plasmidsaurus
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sequences for pyf515 - by Bioz Stars, 2026-06
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Gel electrophoresis of products from a PCR performed on DNA from P. ramorum NA2_17 wildtype (PrWT; wells 3-4), pYF515 transformant (pYF515 PrT; wells 5-7), and pYF2-PsCG transformant (pYF2-PsCG PrT; wells 10-14) cultures using the pYF_Cas9_Diag_F3/R3 primer pair (expected product size is 824 bp). The positive PCR controls are pYF515 plasmid DNA (well 2) and pYF2-PsCG plasmid DNA (well 9). The gel was run at 100 volts for 60 minutes on 0.8% agarose in 0.5X TBE buffer with a 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, MA, USA). All samples were loaded with Safe-Green™ stain (Applied Biological Materials Inc., Richmond, BC, Canada) at a ratio of 1 μL Safe-Green: 5 μL PCR product.

Journal: bioRxiv

Article Title: Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species

doi: 10.1101/2024.06.13.598956

Figure Lengend Snippet: Gel electrophoresis of products from a PCR performed on DNA from P. ramorum NA2_17 wildtype (PrWT; wells 3-4), pYF515 transformant (pYF515 PrT; wells 5-7), and pYF2-PsCG transformant (pYF2-PsCG PrT; wells 10-14) cultures using the pYF_Cas9_Diag_F3/R3 primer pair (expected product size is 824 bp). The positive PCR controls are pYF515 plasmid DNA (well 2) and pYF2-PsCG plasmid DNA (well 9). The gel was run at 100 volts for 60 minutes on 0.8% agarose in 0.5X TBE buffer with a 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, MA, USA). All samples were loaded with Safe-Green™ stain (Applied Biological Materials Inc., Richmond, BC, Canada) at a ratio of 1 μL Safe-Green: 5 μL PCR product.

Article Snippet: The sequencing results from Plasmidsaurus showed the expected sequences for pYF515 (99.8% pairwise identity to original plasmid sequence; S5 Fig) and pYF2-PsCG (99.8% pairwise identity to original plasmid sequence; S6 Fig), but not for either of the two pGFPN samples (52.9% and 62.6% pairwise identity to original plasmid sequence; S7 Fig and S8 Fig, respectively).

Techniques: Nucleic Acid Electrophoresis, Plasmid Preparation, Staining